|
KMID : 0613820070170010162
|
|
Journal of Life Science
2007 Volume.17 No. 1 p.162 ~ p.165
|
|
|
PCR Specific Primer for the Detection of Vibrio tapetis
|
|
Kim Young-Jin
Kim Cheol-Min
Choi Yong-Lark
Park Byoung-Keun
Ahn Soon-Cheol
Lee Sun-Yi
Cho Hyo-Jin
You Sun-Nyung
|
|
|
Abstract
|
|
|
|
Brown Ring Disease (BRD) is a bacterial disease caused by Vibrio tapetis which affects cultured clam Ruditapes philippinarum and causes heavy economic losses on Atlantic coasts of France, Spain and England. In this study, to evaluate the effective detection of the pathogen, specific primer set based on 16S ribosomal RNA (rRNA) sequences designed for rapid detection of V. tapetis. Polymerase chain reaction (PCR) with this primer set produced the specific band for each V. tapetis. The length of PCR product using designed primer set of Vbts-F and Vbts-R was about 400 bp. Therefore, these primers will be provided with a basic tool for rapid detection of V. tapetis in the various cases such as examination of imported aquatic products, diagnosis of aquatic organisms, and etc.
|
|
|
KEYWORD
|
|
|
BRD, Vibrio tapetis, 16S rRNA, PCR, specific primer
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|